Effect of LH on 11β-HSD2 promoter activity. A series of luciferase reporter gene driven by rat 11β-HSD2 promoter region were transfected into mLTC-1 cells. After overnight incubation, the mLTC-1 cells were treated with 20 ng/lm LH for 24 h. The cells were lysed and assayed for luciferase activity. The luciferase activity was compared with the normalized activity of the transfected but not treated cells. These data are representative of at least three independent experiments and are normalized to an internal Renilla control. Asterisks indicate differences between groups control versus LH treatment are statistically significant at P < 0.05.