Figure 3

MTT assay showing proliferative responses. Proliferative responses (stimulation indices) of (A) cervical cells and (B) PBMCs isolated from GI, GII, GIII on stimulation with IncB, IncC and CT EB were estimated by MTT assay. (A) *Significant difference in proliferative responses in GII with respect to GIII (P = 0.0232, P = 0.0297 and P = 0.0400 upon stimulation with IncB, IncC and EB respectively). (B) *Significant difference in proliferative responses in GII with respect to GIII (P = 0.0407, P = 0.0436 and P = 0.0177 upon stimulation with IncB, IncC and CT EBs respectively) where; Group I (GI) comprised of healthy fertile women with no CT infection, Group II (GII) comprised of CT-positive fertile women, Group III (GIII) comprised of CT-positive women with fertility disorders. The horizontal line in the middle of the box is the median value of the responses and the lower (upper) is the 25th (75th) percentile., *Significant; PBMCs- Peripheral blood mononuclear cells; IncB- CT Inclusion membrane protein B; IncC- CT Inclusion membrane protein C; CT- Chlamydia trachomatis; EB- Elementary Bodies; n- Number. Proliferative responses between groups were evaluated using Mann-Whitney U test.