Figure 2

Western blot assay for detection of anti-IncB and anti-IncC IgG antibodies. Detection of (A) anti-IncB IgG and (B) anti-IncC IgG antibodies in sera (S) and cervical washes (CW) obtained from CT-positive patients and controls. Strips 3, 5, 7 – Detection of IncB IgG antibodies in sera (S) of patients' 1, 2, 3 respectively; strips 2, 4, 6- Detection of IncB IgG antibodies in cervical washes (CW) of patients' 1, 2, 3 respectively; strips 1, 2 – Serum and cervical wash respectively obtained from CT-negative patient (which served as negative controls for assay). Strip 9 – Molecular weight marker (Bangalore Genei). A 38 kDa band (IncB specific IgG) and 44.4 kDa band (IncC specific IgG) was detected in sera and cervical washes obtained from CT-positive patients. No bands were observed in negative control. IncB- CT Inclusion membrane protein B; IncC- CT Inclusion membrane protein C; CT- Chlamydia trachomatis; kDa- Kilodaltons; S- Serum; CW- Cervical washes.