Figure 1
From: A novel promoter controls Cyp19a1 gene expression in mouse adipose tissue
The level of Cyp19a1 mRNA in 10-week-old mouse tissues measured by real-time RT-PCR. Cyp19a1 and GAPDH cDNAs from mouse ovary were cloned into pCR®II-TOPO plasmid vector. Different concentrations (10-1 to 10-12 μg DNA/μl) of plasmid DNAs containing Cyp19a1 or GAPDH sequence were amplified with primers for Cyp19a1 or GAPDH as the external standard curves. The ratio of copy numbers of Cyp19a1 (× 107) to copy numbers of GAPDH was calculated as Cyp19a1 mRNA levels. Pituitary was analyzed from pools of 5 animals. Data are expressed as mean ± SE with n = 5 mice in each group.