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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)

Figure 1

A. agrarius express two novel testicular CD46 transcripts with extended 3'- and truncated 5'-untranslated regions. (A) Lane 1, 1000 nt ladder. Lanes 2–4, CD46 exon 5–11 primer set giving no product from A. agrarius or A. sylvaticus testicular RNA, but a 585 bp product from BALB/c testicular RNA. (B) Lane 1, 1000 nt ladder. Lanes 2–4, CD46 exon 5–11 primer set using an A. sylvaticus-specific exon 5 primer giving two products of 509 bp and 564 bp from A. agrarius testicular RNA, and a single 326 bp product from A. sylvaticus testicular RNA, but no product from BALB/c testicular RNA. (C) Lane 1, 1000 nt ladder. Lanes 2–4, 3'-rapid amplification of cDNA ends (3'-RACE) reactions giving products of 204 bp from A. agrarius and A. sylvaticus testicular RNA, and a product of 150 bp from BALB/c testicular RNA. (D) Lane 1, 1000 nt ladder. Lanes 2–4, 5'-RACE reactions giving a product of 353 bp from BALB/c testicular RNA, and various truncated products from A. agrarius and A. sylvaticus testicular RNA. Key: Aa, A. agrarius; As, A. sylvaticus; B, BALB/c.

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