Figure 2

Various K _Ca channels are expressed in human GCs. (A) Cultured GCs express mRNAs encoding SK and IK channels as shown by RT-PCR (top) and cDNA array techniques (bottom). In negative RT-PCR controls (Co) template was replaced by water. (B, C) Expression of SK2 α subunit protein in follicular (B) and luteal GCs (C) of the human ovary. (D) Example of a control experiment (omission of first antiserum) using a slice of the same ovarian sample as in B and E. (E, F) Expression of IK α subunit protein in follicular (E) and luteal GCs (F) of the human ovary. The layer of follicular GCs (*) is delimited by a dotted line (B, D, E). Arrowheads in B label small blood vessels with erythrocytes, which are immunopositive as well. CL, Corpus luteum. Bars, 50 μm.