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Table 1 Number of animals according to age, genotype and vaginal histology.

From: Reduction in corpora lutea number in obese melanocortin-4-receptor-deficient mice

   Age (months)
   3 4 5 6 6
Genotype Estrous cycle number of animals
MC4R+/+ estrus 1 2 1 3 4
  metestrus 3 2 2 2 9
MC4R-/- estrus 2 4 3 4 10
  metestrus 2 0 2 0 3
   1st cohort 2nd cohort
  1. The genotype was identified by PCR analysis as described in Methods. Signs of estrous cycle stage were assigned retrospectively on the basis of vaginal histology. The 1st cohort comprises animals at a mixed C3H/B6 background, the 2nd cohort comprises animals at a B6 background.