Hypertrophy of interstitial gland cells in ovaries of MC4R-deficient mice as measurable decrease in nuclear density. To determine whether hypertrophy of interstitial gland cells (Fig. 1E) occurs to a measurable extent, the nuclear density was determined per 100 μm2-sized areas of interstitial gland cells. Statistically significantly less nuclei are counted in 6-month-old MC4R-deficient C3H/B6 mice than in MC4R+/+ littermates. Data are presented as box-and-whisker plots with outliers; n indicates the number of mice; * highlights significant differences (p ≤ 0.05).