Immunohistochemistry of Hsp105 in rat uterus during early pregnancy. Hsp105 protein was observed mainly in the luminal epithelium on day 1 of pregnancy (D1) and moderately expressed in the luminal epithelium and the glandular epithelium from day 2 to day 3 (D2). Hsp105 staining was also detected in the stromal cells, immediately underneath the luminal epithelium on day 4 and day 5 of pregnancy (D4, D5), the staining was increased markedly on day 5 just before implantation (D5). On day 6, the protein staining was mainly observed in the implanted blastocyst and the stromal cells around the implantation site (D6), while its expression in the luminal epithelium reduced to an undetected level. CON, negtive control; le, luminal epithelium; ge, glandular epithelium; s, stroma; de, decidua; em, embryo. Bar = 200 μm.