|
Primer
|
Sequence
|
Combinations
|
Size (bp)
|
Detects
|
|---|
|
pB5'
|
CCTGAAGTTTCGGCCATACCT
|
p15 & p35
|
284
|
B, A, C, M & S
|
|
pB3'
|
AGCAGTCCGTGTCCTTTCT
|
p33 & p36
|
396
|
B & A
|
|
pS
|
GAATTCAGGAGAGTGGGTGCTC
|
pS & p33
|
186
|
S
|
|
p15
|
AGGAGTTTGTCAAGCTTCAA
|
pB5' & pB3'
|
197
|
B
|
|
p33
|
GAATTCATTTGGAACGCCCACTGG
| | | |
|
p35
|
CTGCAGGGACTGGATAAATGTATTC
| | | |
|
p36
|
CTGCAGGTCTACCCGCCCTATC
| | | |
|
gapdf
|
AGAACATCATCCCTGCCTC
|
gapdf & gapdr
|
347
|
GAPDH
|
|
gapdr
|
GCCAAATTCGTTGTCATACC
| | | |
- Primer combinations, expected amplicon size, reaction conditions and isoforms detected are shown in Fig 2 and are described in references 26 and 33. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.
