The cytoplasmic 60 kDa progesterone receptor isoform predominates in the human amniochorion and placenta at term

Table 1 Designation and sequences of primers used in RT-PCR.

Primer	Sequence	Combinations	Size (bp)	Detects
pB5'	CCTGAAGTTTCGGCCATACCT	p15 & p35	284	B, A, C, M & S
pB3'	AGCAGTCCGTGTCCTTTCT	p33 & p36	396	B & A
pS	GAATTCAGGAGAGTGGGTGCTC	pS & p33	186	S
p15	AGGAGTTTGTCAAGCTTCAA	pB5' & pB3'	197	B
p33	GAATTCATTTGGAACGCCCACTGG
p35	CTGCAGGGACTGGATAAATGTATTC
p36	CTGCAGGTCTACCCGCCCTATC
gapdf	AGAACATCATCCCTGCCTC	gapdf & gapdr	347	GAPDH
gapdr	GCCAAATTCGTTGTCATACC

Primer combinations, expected amplicon size, reaction conditions and isoforms detected are shown in Fig 2 and are described in references 26 and 33. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.

ISSN: 1477-7827