Figure 3From: The cytoplasmic 60 kDa progesterone receptor isoform predominates in the human amniochorion and placenta at termEvidence that the PR isoform in human fetal membranes and placenta at term is either PR-C or PR-M. RT-PCR products generated from fetal membrane samples that had decidua attached (FM + dec) or fetal membranes that had the decidua scraped away (FM – dec) or term placenta (Plac). One μg of DNase-treated total RNA was reverse transcribed in the presence (+), or absence (-), of AMV-RT. Both samples were then amplified in a PCR with isoform-specific primers (see Table 1) that detected all known PR isoforms; the PR-B isoform alone; a combination of PR-A and PR-B; or the PR-S isoform alone. GAPDH was used a control for efficiency of mRNA manipulation and PCR amplification. Myometrium (myo) was used as a positive control for PR with T47D cell extract as a positive control for PR-S (separate column), and in all cases a minus RT sample was incorporated to rule out the presence of contaminating genomic DNA. Results are representative of four independent assessments. Data show the presence of PR in all 4 tissue samples and T47D cells.Back to article page