Figure 3
(A) Mean gel area (cm2) ± SEM (indicated by the error bars) of thrombin treatment of hUtSMC embedded gel time-course experiment (0 to 7 hrs). Significance values are indicated, * P < 0.05, ** P < 0.01, *** P < 0.001. (B) Mean gel area (cm2) ± SEM (indicated by the error bars) of thrombin, hirudin and thrombin plus hirudin treatment of hUtSMC embedded gels at 3 hr post-treatment. Significance values are indicated, * P < 0.05. (C) Graphical representations of real-time fluorescence RT-PCR results of ACTB normalised relative gene expression plotted against control or thrombin treated collagen gel hUtSMC mRNA for F2R ± SEM (indicated by the error bars) performed in triplicate. (D) RNA for the real time fluorescence RT-PCR experiment was isolated from these hUtSMC collagen gels stimulated with 1 U/ml thrombin or control unstimulated gels after 2 hrs. The mean gel area (cm2) ± SEM is indicated by the error bars. Significance values are indicated, ** P < 0.01, *** P < 0.001.