Figure 1

(A) Representative gel pictures of RT-PCR reactions of (i) TAGLN (T) DNA markers (M) PCR water negative (N) are indicated, (ii) ACTB (BA) and CNN1 (C), DNA markers (M) and PCR negative controls (N) for each gene , (iii) ACTA2 (SMA), DNA markers (M), and PCR negative control (N) (iv) ESR1 (E), DNA markers (M) and PCR negative control (N) and (v) PTGS2 (P), DNA markers (M) on RNA isolated from collagen embedded human uterine smooth muscle cells. (B) Representative gel pictures of RT-PCR reactions of (i) TAGLN (T) and PGR (PG), DNA markers (M) and PCR water negatives for both genes (N) respectively, are indicated (ii) ACTB (BA) DNA marker (M) and PCR negative (N) (iii) ACTA2 (BA) DNA marker (M) and PCR negative (N) (iv) CNN1(C) DNA marker and PCR negative (N) (v) PTGS2 (P) DNA marker (M) and PCR negative (N), on RNA isolated from hTERT-HM cells. (C) A visible decrease in gel area of hUtSMC embedded collagen gel lattices is demonstrated as a result of basal contraction, in comparison to no change in gel area with the collagen embedded HEK293 cells.