Immunofluorescent staining of ERα and CD14 observed in the retrodiscal tissue of the TMJ in all four treatment groups. A) No E2/saline, B) No E2/CFA, C) E2/saline, D) E2/CFA. The sections were immunohistochemically stained with mouse anti-ERα and goat polyclonal anti- CD14 primary antibodies followed by anti-mouse Alexa 488 conjugated (green) and anti-goat Alexa 633 conjugated (red) secondary respectively. ERα nuclear with CD14 cytoplasmic staining (arrows), ERα cytoplasmic staining (arrowhead), ERα nuclear staining (open arrowhead) and CD14 staining (open arrow) were observed. Size bar for A, B, D = 47.5 μm.; C = 50 μm.