Excitatory and inhibitory effects of prolactin release activated by nerve stimulation in rat anterior pituitary

Table 1 PRL concentrations of 1-min sampled perfusate, EFS of 2 min (Mean ± SD, pg/ml)

	Group A	Group B	Group C	Group D
-1	117 ± 1.0	118 ± 3.3	111 ± 1.3	107 ± 1.0
0	132 ± 13.0	113 ± 7.4	118 ± 5.7	103 ± 5.1
1	153 ± 15.1 *	115 ± 2.6	134 ±3.3 **	107 ± 1.7
2	184 ± 24.5**	118 ± 4.3	111 ± 6.6	108 ± 3.2
3	214 ± 18.6**	118 ± 1.2	101 ± 6.0	109 ± 1.0
4	214 ± 18.4**	113 ± 4.3	84 ± 3.9**	104 ± 5.8
5	193 ± 32.8**	116 ± 2.6	83 ± 8.6**	107 ± 1.5
6	153 ± 14.9*	115 ± 5.9	93 ± 9.6*	109±2.8
7	131 ± 8.4	117 ± 2.0	105 ± 4.6	108 ± 1.3
8	114 ± 5.3	113 ± 5.0	106 ± 4.6	106 ± 5.3
9	117 ± 3.2	115 ± 3.7	107 ± 1.3	107 ± 1.7
K ⁺	423 ± 42.3**	441 ± 33.4**	382 ± 25.0**	470 ± 35.7**
N	6	5	5	4

Group A: 2 Hz. Group B: 2 Hz + TTX (1 μmol/L). Group C: 10 Hz. Group D: 10 Hz + TTX (1 μmol/L).
The perfusate was continuously collected as aliquots every 1 min and measured by RIA. Italic data represents results during electrical field stimulation.
TTX, tetrodotoxin; EFS, electrical field stimulation; K⁺, prolactin release was evoked by high potassium (60 mmol/L); N, the number of the rat used.
*Significant changes within group (* P < 0.05, ** P < 0.01).

ISSN: 1477-7827