Figure 4

PRL concentrations of sampled perfusate, EFS of 10 Hz for 2 min (Mean ± SD, pg/ml). The parameters of EFS were 20 mA, 0.5 ms and 10 Hz. The perfusate was continuously collected as aliquots and measured by RIA. (1) Perfusate sampled every 10 min (n = 5). (2) Perfusate sampled every 1 min (n = 5). (3) TTX (1 μmol/L) blocking control of (1) (n = 5). (4) TTX (1 μmol/L) blocking control of (2) (n = 4). K⁺, prolactin release was evoked by high potassium (60 mmol/L). * P < 0.05, ** P < 0.01.