Figure 3

Cleavage development of embryos after activation by electroporation (EP), thimerosal/DTT (Thi/DTT), SrCl ₂ and Na⁺-free medium (Na⁺-free) with or without a 4-h incubation in cycloheximide (CHX) and cytochalasin B (Cyto B). The asterisks indicate significant differences (P < 0.05) between cleavage frequencies achieved with and without CHX/Cyto B supplementation. Different lowercase letters indicate significant differences between treatments without CHX/Cyto B supplementation (P < 0.05); different uppercase letters indicate significant differences between treatments where the Ca²⁺ signal-inducing stimulus was followed by CHX/Cyto B incubation (P < 0.05).