Immunolocalization of PPARγ in ovarian tissue sections collected from neonatal rats day 5 (A, D), 7 (B, E), and 11 (C, F) pp (n = 3-4 animals/time point). Tissues were collected and processed as described in the Methods. PPARγ is identified by the brown reaction product. Tissues in D, E, and F were processed with normal goat serum in place of the anti-PPARγ antibody. Arrows in B and C indicate granulosa cells expressing high levels of PPARγ. Original magnification = 200×.