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Table 2 Oligonucleotide sequences used for RT-PCR

From: Human male gamete endocrinology: 1alpha, 25-dihydroxyvitamin D3 (1,25(OH)2D3) regulates different aspects of human sperm biology and metabolism

Gene

Sequence (5' - 3')

Size of PCR product (bp)

VDR

5' - CTCCCCCTGCCAGTGCCTTACCTC - 3'

5' - CCCCGCTCTCCCTTCCCACACT - 3'

299

KIT

5' - AGTACATGGACATGAAACCTGG - 3'

5' - GATTCTGCTCAGACATCGTCG - 3'

780

PTPRC

5' - CAATAGCTACTACTCCATCTAAGCCA - 3'

5' - ATGTCTTATCAGGAGCAGTACATG - 3'

230

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