Figure 4

Molecular characterization of Lichtheimia corymbifera. A) Electrophoresis patterns of amplicons obtained with specific sets of primers for Lichtheimia corymbifera, Rhizopus spp., Rhizomucor spp., Mucor spp. and a set of primers for all other filamentous fungi used as a positive control (840 bp). B) Diagram showing the amplicon (not on scale) and the AclI restriction enzyme predicted fragments of 518 and 306 bp. C) Diagram showing the sub-cloned 824 bp amplicon (not on scale) into the pTZ57R/T vector and AclI restriction sites within the vector (2194 bp and 2567 bp) and within the amplicon. D) Electrophoresis patterns of AclI uncut 824 bp amplicon, AclI cut 824 bp amplicon and AclI cut sub-cloned 824 bp amplicon.