Skip to main content

Table 3 Effects of GDF-8 on hamster blastocysts in culture.

From: Growth-differentiation factor-8 (GDF-8) in the uterus: its identification and functional significance in the golden hamster

Treatment Control GDF-8 (0.75 μg/ml)
No. of Embryos (at 72 hpc) 148 53
Area of embryo image (μm2) 5639.8 ± 125.4 7901.1 ± 317.0***
Perimeter of embryo image (μm) 266.1 ± 2.8 311.8 ± 6.5***
24 h in culture   
No. of embryos reaching late/expanded blastocyst 82 43
No. of trophectoderm (TE) cells 26.9 ± 1.2 37.6 ± 1.4***
No. of Inner Cell Mass (ICM) cells 15.3 ± 0.7 16.9 ± 0.7
TE/ICM 2.1 ± 0.1 2.4 ± 1.1
Total cell number 42.2 ± 1.3 54.5 ± 1.5***
48 h in culture   
No. of embryos 70 50
Area (μm2) 5951.6 ± 218.7 9911.1 ± 648.6***
Perimeter (μm) 280.1 ± 4.8 355.9 ± 10.8***
Hatching   
% embryos hatched (hatched/total No.) 56.9(70/123) 94.3(50/53)++
Attachment   
No. of embryos 69 50
Area (μm2) 21612 ± 1668 12109 ± 1036***
Perimeter (μm) 603.3 ± 36.1 424.7 ± 21.1**
  1. Number of embryos, TE and ICM without treatment were compared with that of treated embryos with Student's t-test; *P < 0.05; **P < 0.01; ***P < 0.001 compared with control; ++P < 0.001 compared with control using χ2 test