Figure 2

Expression profiling of FHL5 mutant (-/-) versus wild-type (+/+) testes. Panel A shows a color-coded heatmap for two groups of genes (up and down-regulated) as indicated by red and blue vertical bars when C57BL/6 & 129/SvPas -/- and B6129SF2/J +/+ were compared. Panel B displays data for these groups obtained in a different sample set as shown. Panel C displays the data obtained with purified testicular cells. The strain background is given at the top for wild-type (+/+) and mutant (-/-) samples. The age distribution of the mice is given. TT is total testis. Samples are in panel A column 1 (KO_09_01, KO_09_02), column 2 (WT_1 and WT_2), panel B column 1 (KO_10_106, KO_10_107, KO_11_91, KO_12_86, KO_12_87), column 2 (KO_16_52, KO_17_60), column 3 (WT_10_105, WT_10_108, WT_11_92) and column 4 (WT_17_61, WT_17_62). Panel C shows duplicate samples of Sertoli cells (SE), spermatogonia (SG), spermatocytes (SC), and spermatids (ST) purified from animals at the appropriate ages [14]. The color code for log2-transformed signals is indicated by a scale to the right of the heatmap ranging from the weakest signal 1 (dark blue) to the strongest signal 15 (dark red).