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Table 4 Monoclonal (mouse) antibodies used for FACS-analysis

From: Specific subsets of immune cells in human decidua differ between normal pregnancy and preeclampsia - a prospective observational study

Antigen

Clone

Isotype

Conjugation

Dilution

Supplier

CD4

RPA-T4

IgG1

PE

1:20

BD-Ph

CD8

RPA-T8

IgG1

PE

1:20

BD-Ph

CD14

M5E2

IgG2a

PE

1:20

BD-Ph

CD16

3G8

IgG1

PE + FITC

1:20

BD-Ph

CD19

HIB19

IgG1

PE

1:20

BD-Ph

CD25

B1.49.9

IgG2a

PE

1:10

BC

CD45

HI30

IgG1

FITC

1:20

BD-Ph

CD56

B159

IgG1

PE

1:20

BD-Ph

Dc-Sign

120507

IgG2b

PE

1:20

RD

HLA-DR

TÜ36

IgG2b

PE

1:20

BD-Ph

ab-T

BMA031

IgG2b

PE

1:10

BC

gd-T

IMMU 510

IgG1

PE

1:10

BC

  1. Antibodies used for flow cytometric analysis of isolated mononuclear decidual cells.
  2. BC: Beckmann Coulter, Krefeld, Germany
  3. BD-Ph: BD Biosciences - Pharmingen, Heidelberg, Germany
  4. RD: R&D-Systems, Heidelberg, Germany