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Table 3 Comparison of the two methods of decidual cell preparation

From: Specific subsets of immune cells in human decidua differ between normal pregnancy and preeclampsia - a prospective observational study

  Decidua (uterus) Decidua (placenta)  
Antigen Median 1. Quart 2. Quart Median 1. Quart 2. Quart p
CD45 (of all cells) 46.1 21.2 54.7 34.0 24.7 41.3 n.s.
Marker on cells in % of CD45+ cells
CD56 52.3 47.3 59.8 42.0 38.9 73.2 n.s.
CD16 25.5 9.2 33.3 17.0 14.0 26.7 n.s.
CD56 + /CD16 + 10.2 8.1 14.3 13.2 6 24.7 n.s.
CD56 ++ /CD16 - 40.9 36.3 52.1 33.4 24.1 38.3 n.s.
HLA-DR 24.2 20.6 32.1 26.4 13.1 30.7 n.s.
DC-Sign 8 6.8 12.1 5.9 3.0 14.7 n.s.
CD14 16.9 8.7 20.2 15.0 12.7 29.2 n.s.
CD8 27.0 23.5 33.5 28.8 23.5 34.9 n.s.
CD4 29.8 22.8 33.6 34.1 28.6 41.7 n.s.
αβ-T 37.5 33.0 45.0 40.5 32.1 46.1 n.s.
γδ-T 3.0 1.6 5.0 2.6 1.3 3.4 n.s.
CD25 5.1 2.7 6.8 3.2 1.9 6.0 n.s.
CD19 1.6 1.2 4 2.9 1.9 3.8 n.s.
  1. Quart = Quartile; 1. Quart: 25%; 2. Quart: 75%. P-values were calculated by the Mann-Whitney-U-Test.
  2. Median, quartiles and p-values of the cell populations examined by FAX analysis comparing decidua obtained from the uterine cavity via curettage and prepared from the placenta (n = 12 in both cases, gestational week: 33-39).
  3. Decidua was obtained either via curettage during caesarean section (attachment site of the placenta plus adjacent decidua) or from the corresponding placenta via preparation of the visible decidua from the basal plate directly after delivery of the placenta. To reduce contamination with peripheral blood cells to a minimum, samples were carefully cleaned of all visible signs of peripheral blood through careful washing. Data are provided as median, quartiles and p-values. NS = difference statistically not significant.