Figure 2

Gene-expression data during postnatal development. Fragments from DDRT-PCR reactions are displayed on long polyacrylamide gels and transcripts identified by sequencing. Intensity of bands was quantified by phosphor imaging. A) Autoradiogram displaying a band corresponding to Dazl, a spermatogonia and spermatocyte-specific gene and below quantification of the band. B) Autoradiogram and quantification of bands corresponding to Vps26a, Gata1 and Ribc2 all generated by the same combination of primers and thus displayed on the same gel. Vps26a and Ribc2 are both examples of genes expressed in pachytene spermatocytes, while Gata1 is expressed in Sertoli cells. C) Autoradiogram and quantification of the spermatid-specific gene, Tnp2.