Figure 3
From: Leukocytospermia and sperm preparation - a flow cytometric study
Pre- (3a) and post-processing (3b, 3c) cytofluorimetric analysis of a semen sample with a moderate leukocyte concentration. Cytogram 3a displays the cytofluorimetric analysis of a semen sample containing a moderate concentration of leukocytes moderate, stained with Syto-16 and CD45 to identify sperm and leukocytes. Region R2 (blue colour) and Region 3 (red colour) represent the number of leukocytes and sperm counted, respectively. Cytograms 3b and 3c displays the cytofluorimetric analysis of the same sample after density-gradient centrifugation and after swim-up technique, respectively.