3A-3J: Immunohistochemistry for detection of ER-β (Figs. 3A-3E) and TUNEL method (Figs. 3F-3J) in the seminiferous tubules affected by cimetidine (CmG). In 3A-3E, spermatocytes (3A), round (3B and 3C), elongate (3E) spermatids and multinucleated cells derived from spermatids (3D) are positive for ER-β immunohistochemistry (arrows). In these cells (3A-3D), the nuclei show degenerative aspect, typical of apoptosis. In 3F-3J, spermatocytes (3F), round (3G), elongate (3J) spermatids and giant multinucleated cells derived from spermatids (3H and 3I) are also TUNEL-positive (arrows). Scale bars: 8 μm.