Figure 2

2A-2I: Immunohistochemistry for detection of ERβ in the testicular sections of rats from CmG. In 2A, the seminiferous tubule showing slight alterations exhibits a similar pattern of immunostaining of CG; however, some spermatid nuclei (white arrows) and the detached germ cells filling the lumen (black arrows) are strongly stained. In the damaged tubules (2B-2E), a strong immunopositivity (black arrows) is observed in the cytoplasm of primary spermatocytes (2B-2D) and germ cells of the basal compartment (2E). Note that the immunolabeling is observed in both cytoplasm (2B) and nucleus (2C) of spermatids (white arrows). In 2F and 2G, strong positive immunolabeling is observed in the nucleus of round (2F) and elongating (2G) spermatids (arrows) and in the residual bodies (asterisks). In 2H, the immunopositive giant multinucleated cells contain spermatids with peripheral condensed chromatin (arrows). In 2I, a positive germ cell is inside a vacuole (arrow) next to Sertoli cell nucleus (S). Scale bars: 28 μm (2A and 2D); 20 μm (2B, 2C, 2E-2I).