Figure 1

1A-1G: Immunohistochemistry for detection of ERβ in the uterus (positive control) and in the testes of rats from CG. In 1A, strong immunolabeling is observed in the epithelium (thin arrow) and endometrial gland (thick arrow). The connective (C) and muscle (M) tissues show a weak and moderate immunopositivity, respectively. In 1B, none immunolabeling is observed in the testicular section used as negative control. In 1C-1G, immunostaining is uniformly observed in the cytoplasm of germ cells of the basal compartment (short arrows), primary spermatocytes (long arrows), round and elongate spermatids (asterisks). An accentuated staining (arrowheads) is observed in the residual bodies (1D) and in the caudal portion of early elongating spermatids (1E). Scale bars: 100 μm (1A); 60 μm (1B); 10 μm (1C); 21 μm (1D and 1E); 15 μm (1F and 1G).