Table 1 The sequence of the designed primers used for RT-PCR
Gene | Genebank | Reverse primer | Forward primer | Product size |
|---|---|---|---|---|
CatSper 1 | AF407332 | 5'CACACACCGGGAATATCTTC3' | 5'TCGGAGAACCACAGAGAAGAG3' | 566 bp |
CatSper 2 | AF411816 | 5'TGTCAGGCTGTTGCTTTGTC3' | 5'TGGCCACAGAGCAGTATTTG3' | 513 bp |
CatSper 3 | AK014942 | 5'GCTCTTCCTCCTCATGTTTG3' | 5'TCTTCCAACATCAGGCTCAG3' | 597 bp |
CatSper 4 | AK077145 | 5'AAGGGGACACAGCAAAGATG3' | 5'TATTCCAGCCATCCTTCCAG3' | 417 bp |
β2m | NM-009735 | 5'TGACCGGCTTGTATGCTATC3' | 5'CACATGTCTCGATCCCAGTAG3' | 316 bp |