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Table 2 Analysis of several cellular and molecular parameters in fresh and frozen/thawed semen collected from three adult male goats (A, B and C).

From: Semen molecular and cellular features: these parameters can reliably predict subsequent ART outcome in a goat model

   A B C
   Fresh Frozen Fresh Frozen Fresh Frozen
Viability (%)   80.4 ± 6.6 60.2 ± 7.9α 74.4 ± 9.1 48.9 ± 11β 75.5 ± 9.7 54.7 ± 11.2α, β
CASA motility parameters* NP motile (%) 58.2 ± 4.9 58.2 ± 14.2 50.5 ± 6.9 57.8 ± 1.6 54 ± 12a 68 ± 8b
  P motile (%) 31.7 ± 5.3 25.7 ± 9.3 32.4 ± 10.7 19.6 ± 11.3 27.2 ± 10.6 20.4 ± 6
  Rapid (%) 65.8 ± 16.4 49.7 ± 15.8 60.8 ± 22.8 45.4 ± 12 56.4 ± 19.2 47.3 ± 4.5
  Medium (%) 12.4 ± 8.6 15.3 ± 5.6 9.6 ± 6.4 13.8 ± 5.3 13.4 ± 10.3 18 ± 3.7
  Slow (%) 11.8 ± 7.7a 18.9 ± 3.1b 12.7 ± 8.5a 23.8 ± 6.1b 13.2 ± 5.7c 22.8 ± 1.8d
  Static (%) 10 ± 5 16.1 ± 17 16.9 ± 12 17 ± 7 16.9 ± 13.4 11.9 ± 3
  VAP (μm/sec) 76.8 ± 23.6 57.9 ± 9.5 69.1 ± 28.9 49.8 ± 12.1 67 ± 23.6 50.5 ± 7.8
  VCL (μm/sec) 105.5 ± 23.4a 83.1 ± 12.1b 96 ± 27.5 74.2 ± 23 94.6 ± 22.3 76.3 ± 9.3
  VSL (μm/sec) 53.5 ± 17.4 41 ± 7.8 50.3 ± 23.5 34.9 ± 14.8 47 ± 18.1 34.2 ± 6.6
  LIN (%) 50.1 ± 9.4 49.7 ± 8.1 50.9 ± 13.7 46.2 ± 8.6 48.7 ± 10.2 44.6 ± 5.6
  STR (%) 69.4 ± 5.4 70.9 ± 6.1 71.5 ± 6.7 69 ± 5.7 69.6 ± 7.3 67.3 ± 4.4
  WOB (%) 71.8 ± 10 69.8 ± 6.1 70.4 ± 14.2 66.5 ± 6.5 69.5 ± 9.6 66.1 ± 4.8
  ALH (μm) 3.3 ± 0.5 3.4 ± 0.5 3.2 ± 0.7 3.6 ± 0.4 3.8 ± 0.6 3.5 ± 0.4
  BCF (Hz) 8.7 ± 1.3 8.2 ± 0.8 9.6 ± 1.9 7.9 ± 1.3 8.9 ± 1.9 7.9 ± 1.4
ATP concentration (nmolATP/109 spermatozoa)   107.4 ± 50.61 48 ± 37 66.5 ± 28.9 27.5 ± 29.5 147.8 ± 55.5a 62.7 ± 45.4b
  1. *P motile: progressive motile sperm; NP motile: non progressive motile sperm; rapid, medium, slow and static: average velocity values of sperm (%); VAP: average path velocity (mm/s); VCL: curvilinear velocity (mm/s); VSL: straight-line velocity (mm/s); LIN: linearity index (%);STR: straightness index (%); ALH: amplitude of lateral head displacement (mm); BCF: beat cross-frequency (Hz); WOB: wobble (%).
  2. α, β Within the same row but among different groups different superscripts indicate statistical difference (ANOVA one way): α ≠ β p < 0.05.
  3. a, b, c, d, Within the same row and group different superscripts indicate statistical difference (ANOVA one way): a ≠ b p < 0.05; c ≠ d p < 0.01.