Figure 5

Representative histochemical localization of Nodal (A-C), Cripto (E-G) and Lefty (I-K) in endometrial carcinomas of Grade 1 (A, E, I), Grade 2 (B, F, J) and Grade 3 (C, G, K). Tumour-associated (T) Nodal and Cripto staining increased in intensity with increasing tumour grade, whereas stromal (S) staining remains relatively unchanged. Negative controls for immunostaining by replacement of antisera with goat IgG at a matching concentration are shown in insets. Bar, 50 μm. Scale bar on (A) relates to panels (A-C & E-G), whereas scale bar on (K) relates to panels (I-K). Tissue sections were also scored according to Nodal (D), Cripto (H) and Lefty (L) staining intensity within endometrial stroma and tumour epithelial cells. Intensity of staining is shown as mean ± SD.