PTX and neomycin do not inhibit the BSA-induced [Ca2+]
change. (A-C) Representative recordings of the [Ca2+]i changes (ΔF/F0) in the sperm head induced by BSA (1 mg/ml) from cells pre-incubated with control (B), PTX (100 ng/ml, 30 min pre-incubation) or neomycin (C) (1 mM, 5 min pre-incubation). (D) Averaged peak ΔF/F0 changes. The numbers of total sperm (from two to three mice) are indicated.