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Table 2 Differences of ROS production in neat semen and in spermatozoa suspension between fertile volunteers and subgroups of males from infertile couples

From: The measurement of reactive oxygen species in human neat semen and in suspended spermatozoa: a comparison

groups

n

ROS (RLU × 103) in neat semen

ap

ROS (RLU × 104) in spermatozoa suspension

bp

FV

34

0.26 (0.12; 0.55)

 

3.5 (1.3; 13.0)

 

NS

39

0.31 (0.12; 1.12)

<0.52

2.4 (1.0; 20.2)

<0.91

SA

52

1.1 (0.19; 4.1)

<0.005

9.3 (1.4; 118.3)

<0.055

SA subgroups of SA EN

27

0.24 (0.15; 1.30)

<0.27

3.9 (0.9; 28.2)

<0.1

SA EL

20

1.14 (0.36; 4.2)

<0.005

11.0 (2.0; 107.3)

<0.76

SA EH

5

32.3 (7.40; 55.8)

<10-5

385.0 (170.0; 625.0)

<10-5

  1. Values are presented as median (25th; 75th percentile). The ap and bp values designate the differences in ROS production in subgroups of males from infertile couples versus FV in neat semen and in spermatozoa in PBS suspension, respectively. A value of p < 0.05 was considered significant by Mann-Whitney U test.
  2. FV - fertile volunteers, NS - normozoospermic males, SA - males with semen abnormalities,
  3. SA EN - subgroup of SA with negative Endtz test, SA EL - subgroup of SA with Endtz test <0.5 × 106/ml, SA EH - subgroup of SA with Endtz test >0.5 × 106/ml
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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