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Table 5 Antibodies used in the study.

From: Prostaglandin treatment is associated with a withdrawal of progesterone and androgen at the receptor level in the uterine cervix

Protein Primary ab, company and order number Type and dilution Inc temp + time Blocking Biotinylated 2 nd Ab all diluted 1:200 Buffer Inc time in RT
PRAB Zymed Lab Inc, 18-0172 Mc mouse anti human 1:150 RT 60 min 1.5% NHS horse anti-mouse 1.5% NHS in PBS 45 min
PRA Novocastra Labs Ltd, NCL-PGR312 Mc mouse anti human 1:500 RT 60 min 1.5% NHS horse anti-mouse 1.5% NHS in PBS 45 min
PRB Affinity BioReagents Inc, MA1-411 Mc mouse Anti chick 1:100 RT 60 min 1.5% NHS horse anti-mouse 1.5% NHS in PBS 45 min
AR DakoCyto-mation Inc, M3562 Mc mouse anti human 1:100 +4°C o/n 2% NHS horse anti-mouse 2% NHS in PBS 30 min
GR Affinity Bio Reagents Inc, PA1-511A Pc rabbit anti human 1:1000 +4°C o/n 2% NGS goat anti rabbit 2% NGS in PBS 60 min
COX-1 Santa Cruz Biotechnology Inc, sc 1752 Pc goat anti human 1:100 +37°C 60 min 10% NRS rabbit anti goat 10% NRS in PBS 30 min
COX-2 Santa Cruz Biotechnology Inc, sc 1745 Pc goat anti human 1:100 +37°C 60 min 10% NRS rabbit anti goat 10% NRS in PBS 30 min
  1. Mc = monoclonal, Pc = polyclonal
  2. RT = room temperature
  3. biotinylated horse anti-mouse (Vector, Cat# BA-2000)
  4. biotinylated goat anti rabbit (Vector, Cat# BA-1000)
  5. biotinylated rabbit anti goat (Vector, Cat# BA-5000)
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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