Table 5 Antibodies used in the study.
Protein | Primary ab, company and order number | Type and dilution | Inc temp + time | Blocking | Biotinylated 2 nd Ab all diluted 1:200 | Buffer | Inc time in RT |
|---|---|---|---|---|---|---|---|
PRAB | Zymed Lab Inc, 18-0172 | Mc mouse anti human 1:150 | RT 60 min | 1.5% NHS | horse anti-mouse | 1.5% NHS in PBS | 45 min |
PRA | Novocastra Labs Ltd, NCL-PGR312 | Mc mouse anti human 1:500 | RT 60 min | 1.5% NHS | horse anti-mouse | 1.5% NHS in PBS | 45 min |
PRB | Affinity BioReagents Inc, MA1-411 | Mc mouse Anti chick 1:100 | RT 60 min | 1.5% NHS | horse anti-mouse | 1.5% NHS in PBS | 45 min |
AR | DakoCyto-mation Inc, M3562 | Mc mouse anti human 1:100 | +4°C o/n | 2% NHS | horse anti-mouse | 2% NHS in PBS | 30 min |
GR | Affinity Bio Reagents Inc, PA1-511A | Pc rabbit anti human 1:1000 | +4°C o/n | 2% NGS | goat anti rabbit | 2% NGS in PBS | 60 min |
COX-1 | Santa Cruz Biotechnology Inc, sc 1752 | Pc goat anti human 1:100 | +37°C 60 min | 10% NRS | rabbit anti goat | 10% NRS in PBS | 30 min |
COX-2 | Santa Cruz Biotechnology Inc, sc 1745 | Pc goat anti human 1:100 | +37°C 60 min | 10% NRS | rabbit anti goat | 10% NRS in PBS | 30 min |