Table 4 Oligonucleotide primers used for real-time PCR.
| Gene | Accession No |
Primers F = forward; R = reverse | Position | cDNA | Annealing step |
|---|---|---|---|---|---|
| PRAB |
NM_000926.4 [37] |
F: tggaagaaatgactgcatcg R: agcatccagtgctctcacaa |
bp 2555-2574 bp 2702-2683 product: 148 bp | 40 ng | 56°C/15 s |
| PRB |
NM_000926.4 [37] |
F: gactgagctgaaggcaaagg R: cgaaacttcaggcaaggtgtc |
bp 746-765 bp 890-870 product: 145 bp | 40 ng | 56°C/15 s |
| AR | NM_000044.2 |
F: taccagctcaccaagctcct R: gcttcactgggtgtggaaat |
bp 3687-3706 bp 3882-3861 product: 195 bp | 100 ng | 56°C/20 s |
| Cyclophilin A | NM_021130.3 |
F: gtggtgtttggcaaagtgaa R: tcgagttgtccacagtcagc |
bp 462-481 bp 577-558 product: 116 bp | 40 ng | 56°C/20 s |