Table 4 Oligonucleotide primers used for real-time PCR.
Gene | Accession No | Primers F = forward; R = reverse | Position | cDNA | Annealing step |
|---|---|---|---|---|---|
PRAB | NM_000926.4 [37] | F: tggaagaaatgactgcatcg R: agcatccagtgctctcacaa | bp 2555-2574 bp 2702-2683 product: 148 bp | 40 ng | 56°C/15 s |
PRB | NM_000926.4 [37] | F: gactgagctgaaggcaaagg R: cgaaacttcaggcaaggtgtc | bp 746-765 bp 890-870 product: 145 bp | 40 ng | 56°C/15 s |
AR | NM_000044.2 | F: taccagctcaccaagctcct R: gcttcactgggtgtggaaat | bp 3687-3706 bp 3882-3861 product: 195 bp | 100 ng | 56°C/20 s |
Cyclophilin A | NM_021130.3 | F: gtggtgtttggcaaagtgaa R: tcgagttgtccacagtcagc | bp 462-481 bp 577-558 product: 116 bp | 40 ng | 56°C/20 s |