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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Leptin interferes with 3',5'-Cyclic Adenosine Monophosphate (cAMP) signaling to inhibit steroidogenesis in human granulosa cells

Figure 2

Leptin inhibited 8-bromo cAMP-stimulated StAR mRNA and protein expression. Cells were treated with leptin (1000 ng/ml) in the presence or absence of 8-bromo cAMP (1 mM) for different time scale (1, 3, 6, 12, 24 h) and total RNA was analyzed with real time RT-PCR to measure the temporal change of StAR mRNA (A), P450scc mRNA (B) and 3β-HSD mRNA (C) levels with GAPDH as internal standard. Cell lysates from cotreatment of leptin with 8-bromo cAMP for 24 h was subjected to Western blot analysis to monitor the protein levels of StAR, P450scc and 3β-HSD (D). The blots are a Western blotting representative data from three independent experiments. Each data point in the figure represents the mean ± SEM of three independent experiments. * above the bars in "A" indicate that cotreatment of leptin with cAMP group significantly differ from cAMP treated group in the particular time scale (P < 0.05). (ctrl = control).

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