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Table 2 Effects of leptin added to IVM medium on equine embryo development

From: Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse

   

N° (%) of oocytes

Cumulus morphology

Leptin concentration (ng/ml)

Evaluated oocytes

N° (%) of 2 cell stage embryos**

N° (%) 4 cell stage embryos*

N° (%) 8 cell stage embryos*

N° (%) 8 cell stage embryos**

Compact

0

40

11 (28)

10 (91)

5 (45)

5 (13)

 

1

20

6 (30)

6 (100)

5 (83)

5 (25)

 

10

26

7 (27)

6 (86)

2 (29)

2 (8)

 

100

25

5 (20)

5 (100)

0 (0)

0 (0)

 

1000

26

5 (19)

4 (80)

0 (0)

0 (0)

Expanded

0

39

8 (21)

7 (88)

5 (63)

5 (13)

 

1

23

8 (35)

8 (100)

5 (63)

5 (22)

 

10

16

6 (38)

5 (83)

4 (67)

4 (25)

 

100

23

6 (26)

4 (67)

1 (17)

1 (4)

 

1000

24

9 (38)

7 (78)

2 (22)

2 (8)

Total

0

79

19 (24)

17 (89)

10 (53)a

10 (13)

 

1

43

14 (33)

14 (100)

10 (71)

10 (23)

 

10

42

13 (31)

11 (85)

6 (46)

6 (14)

 

100

48

11 (23)

9 (82)

1 (9)b

1 (2)

 

1000

50

14 (28)

11 (79)

2 (14)

2 (4)

  1. (*) percentages of the two cell-stage embryos
  2. (**) percentages of the evaluated oocytes
  3. Different superscripts indicate statistically differences at P < 0.05 (Chi-square-test).
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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