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Table 2 Effects of leptin added to IVM medium on equine embryo development

From: Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse

    N° (%) of oocytes
Cumulus morphology Leptin concentration (ng/ml) Evaluated oocytes N° (%) of 2 cell stage embryos** N° (%) 4 cell stage embryos* N° (%) 8 cell stage embryos* N° (%) 8 cell stage embryos**
Compact 0 40 11 (28) 10 (91) 5 (45) 5 (13)
  1 20 6 (30) 6 (100) 5 (83) 5 (25)
  10 26 7 (27) 6 (86) 2 (29) 2 (8)
  100 25 5 (20) 5 (100) 0 (0) 0 (0)
  1000 26 5 (19) 4 (80) 0 (0) 0 (0)
Expanded 0 39 8 (21) 7 (88) 5 (63) 5 (13)
  1 23 8 (35) 8 (100) 5 (63) 5 (22)
  10 16 6 (38) 5 (83) 4 (67) 4 (25)
  100 23 6 (26) 4 (67) 1 (17) 1 (4)
  1000 24 9 (38) 7 (78) 2 (22) 2 (8)
Total 0 79 19 (24) 17 (89) 10 (53)a 10 (13)
  1 43 14 (33) 14 (100) 10 (71) 10 (23)
  10 42 13 (31) 11 (85) 6 (46) 6 (14)
  100 48 11 (23) 9 (82) 1 (9)b 1 (2)
  1000 50 14 (28) 11 (79) 2 (14) 2 (4)
  1. (*) percentages of the two cell-stage embryos
  2. (**) percentages of the evaluated oocytes
  3. Different superscripts indicate statistically differences at P < 0.05 (Chi-square-test).