Table 1 Dose response effect of leptin on in vitro maturation and fertilization rates of equine oocytes after ICSI
|  |  |  |  | N° (%) of oocytes |  |  | |
|---|---|---|---|---|---|---|---|
Cumulus morphology | Leptin concentration (ng/ml) | Evaluated | MII+PB and injected* | Normally fertilized* | Abnormally fertilized* | Total fertilization* | Activated* |
Compact | 0 | 40 | 17 (43) | 11 (28) | 0 (0) | 11 (28) | 0 (0) |
| Â | 1 | 20 | 11 (55) | 6 (30) | 0 (0) | 6 (30) | 1 (5) |
| Â | 10 | 26 | 13 (50) | 8 (31) | 0 (0) | 8 (31) | 1 (4) |
| Â | 100 | 25 | 9 (36) | 5 (20) | 1 (4) | 6 (24) | 1 (4) |
| Â | 1000 | 26 | 12 (46) | 5 (19) | 1 (4) | 6 (23) | 1 (4) |
Expanded | 0 | 39 | 17 (44)a | 9 (23) | 0 (0) | 9 (23)a | 2 (5) |
| Â | 1 | 23 | 14 (61) | 8 (35) | 0 (0) | 8 (35) | 0 (0) |
| Â | 10 | 16 | 12 (75) | 8 (50) | 1 (6) | 9 (56)b | 0 (0) |
| Â | 100 | 23 | 17 (74)b | 7 (30) | 2 (9) | 9 (39) | 1 (4) |
| Â | 1000 | 24 | 17 (71) | 9 (38) | 1 (4) | 10 (42) | 0 (0) |
Total | 0 | 79 | 34 (43) | 20 (25) | 0 (0) | 20 (25) | 2 (3) |
| Â | 1 | 43 | 25 (58) | 14 (33) | 0 (0) | 14 (33) | 1 (2) |
| Â | 10 | 42 | 25 (60) | 16 (38) | 1 (2) | 17 (40) | 1 (2) |
| Â | 100 | 48 | 26 (54) | 12 (25) | 3 (6) | 15 (31) | 2 (4) |
| Â | 1000 | 50 | 29 (58) | 14 (28) | 2 (4) | 16 (32) | 1 (2) |