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Table 1 Dose response effect of leptin on in vitro maturation and fertilization rates of equine oocytes after ICSI

From: Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse

     N° (%) of oocytes   
Cumulus morphology Leptin concentration (ng/ml) Evaluated MII+PB and injected* Normally fertilized* Abnormally fertilized* Total fertilization* Activated*
Compact 0 40 17 (43) 11 (28) 0 (0) 11 (28) 0 (0)
  1 20 11 (55) 6 (30) 0 (0) 6 (30) 1 (5)
  10 26 13 (50) 8 (31) 0 (0) 8 (31) 1 (4)
  100 25 9 (36) 5 (20) 1 (4) 6 (24) 1 (4)
  1000 26 12 (46) 5 (19) 1 (4) 6 (23) 1 (4)
Expanded 0 39 17 (44)a 9 (23) 0 (0) 9 (23)a 2 (5)
  1 23 14 (61) 8 (35) 0 (0) 8 (35) 0 (0)
  10 16 12 (75) 8 (50) 1 (6) 9 (56)b 0 (0)
  100 23 17 (74)b 7 (30) 2 (9) 9 (39) 1 (4)
  1000 24 17 (71) 9 (38) 1 (4) 10 (42) 0 (0)
Total 0 79 34 (43) 20 (25) 0 (0) 20 (25) 2 (3)
  1 43 25 (58) 14 (33) 0 (0) 14 (33) 1 (2)
  10 42 25 (60) 16 (38) 1 (2) 17 (40) 1 (2)
  100 48 26 (54) 12 (25) 3 (6) 15 (31) 2 (4)
  1000 50 29 (58) 14 (28) 2 (4) 16 (32) 1 (2)
  1. (*) percentages of the evaluated oocytes
  2. Percentages with different superscripts statistically differ at P < 0.05 (Chi-square-test).