Early hCG addition to rFSH for ovarian stimulation in IVF provides better results and the cDNA copies of the hCG receptor may be an indicator of successful stimulation

Table 1 PCR Primers

mRNA	PCR primer pair	Primers 5'-3'	Sequence	Annealing Temperature (°C)	Product size
LH/hCG	Outer pair	Forward	CAA TGT GAA AGC ACA GTA AGG A	56
		Reverse	AGG CTA TGA GCA GCA GAT AGA G	56	343
	Inner pair	Forward	GAA CTG AGT GGC TGG GAC TA	56
		Reverse	GCA AAA GTC TGC AAA GGA GA	56	249
HPRT	Outer pair	Forward	CTCCGCCTCCTCCTCTGCT	50
		Reverse	GCCTGACCAAGGAAAGCAAAG	50	528
		Forward	GCCGGCTCCGTTATGGCG	55
	Inner pair	Reverse	AGCCCCCCTTGAGCACACAGA	55	226

ISSN: 1477-7827