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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients

Figure 5

Effects of cytochalasin D and jasplakinolide on progesterone induced calcium entry in human spermatozoa from asthenozoospermic patients. (A) Fura-2-loaded human spermatozoa were preincubated at room temperature in the presence of 10 μM cytochalasin D (Cyt D) for 40 min or 10 μM jasplakinolide (JP) for 30 min. Cells were then stimulated with 20 μM progesterone (PROG) plus 1 μM thapsigargin (TG) in calcium-free medium (+ 100 μM EGTA), and 6 min later CaCl2 (300 μM) was added to the medium to initiate calcium entry. Traces are representative of seven independent experiments. (B) Histogram represents the integral for 2.5 min of the amount of calcium entry, in control, Cyt D-treated and JP-treated spermatozoa, calculated as described in Methods section. Values are means ± SD of seven independent experiments.

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