Figure 3
From: Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients
Progesterone induced calcium entry in human spermatozoa from normospermic or asthenozoospermic patients. (A) Fura-2-loaded human spermatozoa were treated with 20 μM progesterone (PROG) plus 1 μM thapsigargin (TG) for 6 min in a calcium-free medium (+ 100 μM EGTA) followed by addition of CaCl2 (300 μM) to initiate calcium entry. Traces are representative of seven independent experiments. (B) Histogram represents the integral for 2.5 min of the amount of calcium entry, in normospermic and asthenozoospermic patients, calculated as described in Methods section. Values are means ± SD of seven independent experiments. *P < 0.05.