Figure 2
From: Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients
Effect of the blockade of progesterone receptor on calcium mobilization evoked by progesterone in human spermatozoa. (A) Fura-2-loaded human spermatozoa from normospermic and asthenozoospermic patients were stimulated with 20 μM progesterone (PROG) plus 1 μM thapsigargin (TG) in a calcium-normal solution (1.2 mM [Ca2+]0). (B) Fura-2-loaded human spermatozoa from normospermic patients were pretreated with the anti-progesterone receptor c262 antibody (PR c262) (1:10, final concentration 100 μg/ml for 30 min) or the progesterone receptor antagonist RU-38486 (50 μM for 30 min) and then stimulated with 20 μM progesterone (PROG) in a calcium-normal solution (1.2 mM [Ca2+]0). Traces are representative of 3–4 independent experiments.