Figure 1
From: Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients
Mobilization of calcium in response to progesterone in human spermatozoa from normospermic or asthenozoospermic patients. Fura-2-loaded human spermatozoa from normospermic (A) or asthenozoospermic (B) patients were stimulated with 20 μM progesterone alone (PROG) (insets) or plus 1 μM thapsigargin (TG) in calcium-free solution (+ 1 mM EGTA), in the absence (control) or presence of dimethyl BAPTA (10 μM for 30 min). Traces are representative of five independent experiments. (C) Histogram represents the integral for 2.5 min of the calcium release, in normospermic and asthenozoospermic patients, calculated as described in Methods section. Values are means ± SD of five independent experiments. *P < 0.05.