Figure 1

Subcellular localization of ER-α36. Hec1A cells were fixed and immunofluorescently stained with the anti-ER-α36 specific antibody against the 20 unique amino acids at the C-terminal of ER-α36 (green). The cells were counterstained with Hoechst 33258 (blue) to show the cell nuclei (A). Total protein was extracted from MCF-7, Hec1A and LNCaP cells and expression of ER-α66 (B) and androgen receptor (AR) (C) was analyzed by Western blot.