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Table 2 Localization of PS on spermatozoa labeled by annexin V-FITC in DGC-prepared sperm

From: Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential

PS localization DGCprepared sperm
Noncapacitated Sperm
(n = 29) DGC-prepared sperm
Capacitated Sperm
(n = 29)
Total (%A-) + (%A+) 100   100  
Annexin V- (%A-) 86.7 ± 1.8 (61–98)   83.7 ± 1.8 (55–98)  
Annexin V+ (%A+) 13.3 ± 1.8 (2–39) 100 16.3 ± 1.8 (2–45)S 100
Head   14.7 ± 2.2 (0–46)   18.6 ± 2.0 (0–57)S
Head and Midpiece   11.5 ± 1.9 (0–38)   15.4 ± 1.8 (0–44)S
Midpiece   7.7 ± 1.4 (0–27)   7.8 ± 1.3 (0–22) NS
Tail   4.7 ± 1.2 (0–33)   5.1 ± 1.4 (0–33) NS
Entire spermatozoon   61.4 ± 3.6 (25–100)   53.1 ± 3.5 (18–89)S
  1. Values are mean percentages ± SEM (minimum-maximum values).
  2. NS = statistically not significant compared to noncapacitated sperm (paired t-test).
  3. S = statistically significant compared to noncapacitated sperm with p < 0.05 (paired t-test)