Table 2 Localization of PS on spermatozoa labeled by annexin V-FITC in DGC-prepared sperm

Total (%A-) + (%A+)

100

100

Annexin V- (%A-)

86.7 ± 1.8 (61–98)

83.7 ± 1.8 (55–98)

Annexin V+ (%A+)

13.3 ± 1.8 (2–39)

100

16.3 ± 1.8 (2–45)^S

100

Head

14.7 ± 2.2 (0–46)

18.6 ± 2.0 (0–57)^S

Head and Midpiece

11.5 ± 1.9 (0–38)

15.4 ± 1.8 (0–44)^S

Midpiece

7.7 ± 1.4 (0–27)

7.8 ± 1.3 (0–22) ^NS

Tail

4.7 ± 1.2 (0–33)

5.1 ± 1.4 (0–33) ^NS

Entire spermatozoon

61.4 ± 3.6 (25–100)

53.1 ± 3.5 (18–89)^S