Skip to main content

Table 1 Identification and quantification of EPS-positive spermatozoa by Annexin V-FITC labeling in DGC-prepared sperm.

From: Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential

Subpopulations of spermatozoa DGC-prepared sperm
Noncapacitated sperm (n = 17)
DGC-prepared sperm
Capacitated sperm (n = 17)
Annexin V-/Hoechst - (%A-/H-) 70.2 ± 5.8 (18–96) 66.2 ± 5.3 (25–91)S
Annexin V-/Hoechst + (%A-/H+) 15.7 ± 4.1 (1–52) 13.8 ± 3.6 (2–52) NS
Annexin V+/Hoechst - (%A+/H-) 2.9 ± 0.8 (0–15) 3.6 ± 0.6 (1–8) NS
Annexin V+/Hoechst + (%A+/H+) 11.2 ± 2.4 (1–34) 16.4 ± 2.7 (4–39)S
Total Annexin V + (%A+)
(%A+/H-)+(%A+/H+)
14.2 ± 2.7 (2–38) 20.1 ± 2.8 (5–41) S
Apoptotic index (A+/H-)/total (H-) 4.5 ± 1.2 (0–22) 5.7 ± 0.9 (1–11) NS
  1. values are mean percentages ± SEM (minimum-maximum values).
  2. NS = statistically not significant compared to noncapacitated sperm (paired t-test).
  3. S = statistically significant compared to noncapacitated sperm with p < 0.05 (paired t-test).