Table 1 Identification and quantification of EPS-positive spermatozoa by Annexin V-FITC labeling in DGC-prepared sperm.
Subpopulations of spermatozoa | DGC-prepared sperm Noncapacitated sperm (n = 17) | DGC-prepared sperm Capacitated sperm (n = 17) |
|---|---|---|
Annexin V-/Hoechst - (%A-/H-) | 70.2 ± 5.8 (18–96) | 66.2 ± 5.3 (25–91)S |
Annexin V-/Hoechst + (%A-/H+) | 15.7 ± 4.1 (1–52) | 13.8 ± 3.6 (2–52) NS |
Annexin V+/Hoechst - (%A+/H-) | 2.9 ± 0.8 (0–15) | 3.6 ± 0.6 (1–8) NS |
Annexin V+/Hoechst + (%A+/H+) | 11.2 ± 2.4 (1–34) | 16.4 ± 2.7 (4–39)S |
Total Annexin V + (%A+) (%A+/H-)+(%A+/H+) | 14.2 ± 2.7 (2–38) | 20.1 ± 2.8 (5–41) S |
Apoptotic index (A+/H-)/total (H-) | 4.5 ± 1.2 (0–22) | 5.7 ± 0.9 (1–11) NS |