Figure 5From: Epidermal growth factor mediates spermatogonial proliferation in newt testisDose-dependent effects of inhibitors for MAPK and PI3K on EGF-stimulated spermatogonial proliferation. Testicular fragments containing spermatogonial stage treated without (0) or with various doses of a MAPK-specific inhibitor (PD98059) (A) or PI3K-specific inhibitors (Wortmannin (B) and LY294002 (C)), were cultured for 1 week in the absence (Control) or presence of either FSH (200 ng/μl) or EGF (4000 ng/μl), followed by BrdU incorporation assay. *, P < 0.05; **, P < 0.01.Back to article page