Figure 4
From: Epidermal growth factor mediates spermatogonial proliferation in newt testis
Dose-dependent effects of inhibitors for ErbB on EGF-stimulated spermatogonial proliferation. Testicular fragments containing spermatogonial stage treated without (0) or with various doses of pan ErbB4 inhibitor (PD153035) (A), an ErbB2-specific inhibitor (AG879) (B), or an ErbB1-specific inhibitor (AG1478) (C) were cultured for 1 week in the absence (Control) or presence of either FSH (200 ng/μl) or EGF (4000 ng/μl), followed by BrdU incorporation assay. *, P < 0.05; **, P < 0.01.