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Table 1 Tissue collection schedule.

From: Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium

Symbol of group

Actual collection days

No. of animals

Definition for purposes

Estrous cycle

   

E0

day 0

2

QPCR, in situ hybridization

E7

day 7

2

QPCR

E13

day 13

2

microarray

Gestation

   

Day 15

day 15*,19, 21*

3

QPCR, *zymography (in situ)

Day 21

day 21

1

in situ hybridization

Day 25

day 25

2

microarray

 

day 25, 32

2

zymography (extraction)

Day 30

day 27, 30, 34

3

QPCR

Day 60

day 56, 56, 60, 64

4

QPCR, microarray

Day 150

day 97, 148, 150

3

QPCR, microarray

 

day 144, 148,150

3

zymography (extraction)

Day 250

day 245, 249, 252

3

QPCR, microarray, zymography (extraction)

  1. QPCR: Real-time RT-PCR. *: Each of them was used for in situ zymography as shown Day 15 and Day 21 in Figure 4.